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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
04/08/2022 |
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Data da última atualização: |
04/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, P. A.; TROBO-MASEDA, L.; LIMA, F. A.; MORAIS JÚNIOR, W. G. DE; MARCO, J. LISBOA DE; SALUM, T. F. C.; GUISAN, J. M. |
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Afiliação: |
PEDRO ALVES MARTINS, Universidade de Brasília; LARA TROBO-MASEDA, Instituto de Catalisis y Petroleoquímica, Madrid, Spain; FREDERICO ALVES LIMA, Instituto de Catalisis y Petroleoquímica, Madrid, Spain; WILSON GALVAO DE MORAIS JÚNIOR, Instituto de Catalisis y Petroleoquímica, Madrid, Spain; JANICE LISBOA DE MARCO, Universidade de Brasília; THAIS FABIANA CHAN SALUM, CNPAE; JOSE MANUEL GUISAN, Instituto de Catalisis y Petroleoquímica, Madrid, Spain. |
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Título: |
Omega-3 production by fish oil hydrolysis using a lipase from Burkholderia gladioli BRM58833 immobilized and stabilized by post-immobilization techniques. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biochemistry and Biophysics Reports, v. 29, 101193, 2022. |
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Idioma: |
Português |
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Conteúdo: |
Immobilization of lipase from Burkholderia gladioli BRM58833 on octyl sepharose (OCT) resulted in catalysts with higher activity and stability. Following, strategies were studied to further stabilize and secure the enzyme to the support using functionalized polymers, like polyethylenimine (PEI) and aldehyde-dextran (DEXa), to cover the catalyst with layers at different combinations. Alternatively, the construction of a bifunctional layer was studied using methoxypolyethylene glycol amine (NH 2 -PEG) and glycine. The catalyst OCT-PEI-DEXa was the most thermostable, with a 263.8-fold increase in stability when compared to the control condition. When evaluated under alkaline conditions, OCT-DEXa-PEG 10 /Gly was the most stable, reaching stability 70.1 times greater than the control condition. Proportionally, the stabilization obtained for B. gladioli BRM58833 lipase was superior to that obtained for the commercial B. cepacia lipase. Preliminary results in the hydrolysis of fish oil demonstrated the potential of the coating technique with bifunctional polymers, resulting in a stable catalyst with greater catalytic capacity for the production of omega-3 PUFAs. According to the results obtained, it is possible to modulate B. gladioli BRM58833 lipase properties like stability and catalytic activity for enrichment of omega-3 fatty acids. |
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Palavras-Chave: |
Enzyme stabilization. |
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Thesagro: |
Lípase. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145248/1/SALUM-et-al.-Artigo-SI-12.15.00.007.00.05.001.pdf
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Marc: |
LEADER 02071naa a2200217 a 4500
001 2145248
005 2022-08-04
008 2022 bl uuuu u00u1 u #d
100 1 $aMARTINS, P. A.
245 $aOmega-3 production by fish oil hydrolysis using a lipase from Burkholderia gladioli BRM58833 immobilized and stabilized by post-immobilization techniques.$h[electronic resource]
260 $c2022
520 $aImmobilization of lipase from Burkholderia gladioli BRM58833 on octyl sepharose (OCT) resulted in catalysts with higher activity and stability. Following, strategies were studied to further stabilize and secure the enzyme to the support using functionalized polymers, like polyethylenimine (PEI) and aldehyde-dextran (DEXa), to cover the catalyst with layers at different combinations. Alternatively, the construction of a bifunctional layer was studied using methoxypolyethylene glycol amine (NH 2 -PEG) and glycine. The catalyst OCT-PEI-DEXa was the most thermostable, with a 263.8-fold increase in stability when compared to the control condition. When evaluated under alkaline conditions, OCT-DEXa-PEG 10 /Gly was the most stable, reaching stability 70.1 times greater than the control condition. Proportionally, the stabilization obtained for B. gladioli BRM58833 lipase was superior to that obtained for the commercial B. cepacia lipase. Preliminary results in the hydrolysis of fish oil demonstrated the potential of the coating technique with bifunctional polymers, resulting in a stable catalyst with greater catalytic capacity for the production of omega-3 PUFAs. According to the results obtained, it is possible to modulate B. gladioli BRM58833 lipase properties like stability and catalytic activity for enrichment of omega-3 fatty acids.
650 $aLípase
653 $aEnzyme stabilization
700 1 $aTROBO-MASEDA, L.
700 1 $aLIMA, F. A.
700 1 $aMORAIS JÚNIOR, W. G. DE
700 1 $aMARCO, J. LISBOA DE
700 1 $aSALUM, T. F. C.
700 1 $aGUISAN, J. M.
773 $tBiochemistry and Biophysics Reports$gv. 29, 101193, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/02/2024 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CARVALHO, C. V. de; COUTINHO, C. de C.; HONÓRIO, N. T. de B. S.; NOGUEIRA, A. L.; FERREIRA, R. C.; LIMA, L. L.; JESUS, L. M. de; MAGALHÃES, R. L. O. de; SILVA, J. M. A. da; PEREIRA, H. P.; DOMINGUES, R.; REIS, D. R. de L.; MACHADO, M. A.; MARTINS, M. F.; GASPAR, E. B. |
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Afiliação: |
CLARISSA VIDAL DE CARVALHO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; CINTHIA DE CARVALHO COUTINHO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; NICOLE TAFNES DE BRITO SILVA HONÓRIO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ARIANY LACERDA NOGUEIRA, UNIVERSIDADE PRESIDENTE ANTÔNIO CARLOS; RAÍSSA CURY FERREIRA, CENTRO UNIVERSITÁRIO DO SUDESTE MINEIRO; LIDIANE LOEFFLER LIMA, CENTRO DE EDUCAÇÃO SUPERIOR DE JUIZ DE FORA; LETÍCIA MILENA DE JESUS, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; RAFAELLA LIMA OLIVEIRA DE MAGALHÃES, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JOÃO MARCOS ALVES DA SILVA; HYAGO PASSE PEREIRA; ROBERT DOMINGUES, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MARTA FONSECA MARTINS, CNPGL; EMANUELLE BALDO GASPAR, CNPGL. |
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Título: |
Isolamento de espécies de Moraxella em um surto de Ceratoconjuntivite Infecciosa em Minas Gerais. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: CONGRESSO DE MICROBIOLOGIA, 3., 2023, Juiz de fora. Doenças infecciosas emergentes: resumos. Universidade Federal de Juiz de Fora, 2023. |
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Idioma: |
Português |
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Palavras-Chave: |
Ceratoconjuntivite infecciosa. |
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Thesagro: |
Bactéria; Bovino; Doença Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161866/1/Isolamento-de-espEcies-de-Moraxella.pdf
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Marc: |
LEADER 01040nam a2200313 a 4500
001 2161866
005 2024-04-10
008 2023 bl uuuu u00u1 u #d
100 1 $aCARVALHO, C. V. de
245 $aIsolamento de espécies de Moraxella em um surto de Ceratoconjuntivite Infecciosa em Minas Gerais.$h[electronic resource]
260 $aIn: CONGRESSO DE MICROBIOLOGIA, 3., 2023, Juiz de fora. Doenças infecciosas emergentes: resumos. Universidade Federal de Juiz de Fora$c2023
650 $aBactéria
650 $aBovino
650 $aDoença Animal
653 $aCeratoconjuntivite infecciosa
700 1 $aCOUTINHO, C. de C.
700 1 $aHONÓRIO, N. T. de B. S.
700 1 $aNOGUEIRA, A. L.
700 1 $aFERREIRA, R. C.
700 1 $aLIMA, L. L.
700 1 $aJESUS, L. M. de
700 1 $aMAGALHÃES, R. L. O. de
700 1 $aSILVA, J. M. A. da
700 1 $aPEREIRA, H. P.
700 1 $aDOMINGUES, R.
700 1 $aREIS, D. R. de L.
700 1 $aMACHADO, M. A.
700 1 $aMARTINS, M. F.
700 1 $aGASPAR, E. B.
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Embrapa Gado de Leite (CNPGL) |
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